FIGURE 2.

Viral vector-mediated A53T α-synuclein overexpression in the substantia nigra does not affect P2X7 availability. (A) Representative images of [¹¹C]JNJ-717 autoradiograms across all time points obtained from rats injected with viral vector overexpressing A53T mutant α-synuclein in the substantia nigra. Images of respective blocking studies and eGFP controls at the 4-week time point were included. (B) Ipsi- to contralateral ratios of tracer binding in the regions-of-interest are represented in box plots. No significant changes were found. The gray lines indicate the theoretical ipsi- to contralateral binding ratio in healthy rats (∼1). N = 4–7 per group and per time point with ≥4 analyzed brain slices per animal. 2-way ANOVA with Sidak post hoc test. IL, ipsilateral; CL, contralateral; DLU, digital light units; L, left; R, right; eGFP, enhanced green fluorescent protein; α-SYN, α-synuclein.