Figure 2.

Evaluation of printing parameters after conversion of the off-the-shelf printer into a 3D bioprinter. (A,B) Representative microscopic images of bromophenol blue-5% alginate hydrogel (A) and 5% alginate/5% gelatin hydrogel (B) lines, printed onto a glass coverslip and captured using a 4x objective. Scale bar: 1 mm. (C) Histogram of the width (in μm) of the printed lines (mean ± SD) for evaluation of the printing accuracy and repeatability for 5% alginate and a mixture of alginate and gelatin. The dotted line represents the target line width of 800 μm. These data demonstrate the suitability of the ultra-low-cost 3D bioprinter to printing of different hydrogels and indicate moderate to high accuracy and reproducibility.