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. 2019 Jul 31;7:184. doi: 10.3389/fbioe.2019.00184

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Copyright © 2019 Kahl, Gertig, Hoyer, Friedrich and Gilbert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maturation of 2D printed recombinant HEK293^YFP cell-laden alginate-hydrogel using the ultra-low-cost system. (A) Reconstructed and registered fluo-micrographs of the printed structure shown in Figure 2A after 0, 2, 5, and 7 days in vitro, respectively, indicating cellular proliferation and viability within the bioconstruct. These data demonstrate the suitability of the in house-built system for ultra-low-cost bioprinting. Scale bar: 2 mm. (B) Time-course of average relative fluorescence intensity (RFU, mean ± SD) during long-term culture as indicator of cellular proliferation of HEK293^YFP cells. The dotted line represents an exponential fit indicating exponential growth of the cells within the hydrogel and high cellular viability. (C) 3D bioprinting in our lab during operation. Due to its light-weight and small dimensions, the device can be operated within a standard laminar flow hood under sterile conditions. Written informed consent was obtained from the individual for the publication of this image.