Figure 2.

Effects of SAMDC1 overexpression on ABA metabolism and response. Four-week old Arabidopsis WT and pBISDCs transgenic plants overexpressing SAMDC1 (S3′, S9, and S15), were watered with solution without (control) or with 250 mM NaCl (saline stress). Expression levels of a set of ABA biosynthesis and ABA-response related genes were determined in leaves by qRT-PCR after 6 h in control (A) or salt stress conditions (B). For each gene, data are expressed as fold change relative to the level measured in WT plants in control conditions (2^−ΔΔCT). ABA levels were also determined in the same leaves in control (C) and salt stress conditions (D), expressed as ng (g FW)⁻¹. Graph show the mean of three biological replicates ± standard deviation. Significant differences between plant lines are indicated with letters (ANOVA, Tukey HSD test, p < 0.05).