Figure 3.
Effects of external PA treatment on ABA levels and ABA-related gene expression. Arabidopsis WT plants were grown for 5 days in plates supplemented with Put, Spd, or Spm, as well as in control plates without the amendment of PAs. (A) ABA levels, expressed as ng (g FW)−1. (B) Expression levels of ABA-responsive genes were determined by qRT-PCR. For each gene, data are expressed as fold change relative to the level measured in WT plants in control conditions (2−ΔΔCT). Graph show the mean of three biological replicates ± standard deviation. Significant differences between treatments are indicated with letters (ANOVA, Tukey HSD test, p < 0.05).