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. 2019 Jul 31;10:972. doi: 10.3389/fpls.2019.00972

Figure 5.

Figure 5

Effects of Spm levels, Spm treatment or tungstate treatment on ABA levels and gene expression of ABA-related genes. Arabidopsis WT, and pBISDCs transgenic plants overexpressing SAMDC1 (S3′, S9, and S15) plants were grown for 5 days in plates without (control), or supplemented with 0.1 mM tungstate (tungstate). Additionally, WT plants were grown in plates supplemented with 0.5 mM SPM. ABA levels (A), expressed as ng (g FW)−1, were measured for each plant and condition. Also, qRT-PCR was used to determine the expression levels of ABA-related genes in all conditions assayed (B–E). For each gene, data are expressed as fold change relative to the level measured in WT plants in control conditions (2−ΔΔCT). Graph show the mean of three biological replicates ± standard deviation. Significant differences between treatments are indicated with letters (ANOVA, Tukey HSD test, p < 0.05).