Table 1.
Biological samples collected, laboratory tests performed, and pathogens investigated, during an integrated surveillance program for zoonoses in western Kenya.
| Biological sample | Laboratory test | Pathogen investigated | Protocol |
|---|---|---|---|
| Feces | Bacterial culture and identification | E. coli | Table S8 |
| Salmonella spp. | Table S9 | ||
| Campylobacter spp. | Table S10 | ||
| Mesenteric lymph nodes (pigs) | Bacterial culture and identification | Salmonella spp. | Table S9 |
| Campylobacter spp. | Table S10 | ||
| Nasal swabs | Bacterial culture and identification | Staphylococcus aureus | Table S11 |
| Blood | |||
| – Serum | Rose Bengal Test | Brucella spp. | Table S12 |
| ELISA | Coxiella burnetii (Human anti-Q-Ab ELISA Kit [Biosource] and Ruminant CHECKIT Q Fever Ab Test [IDEXX]) | As per manufacturer's instructions | |
| Rift Valley Fever virus (ID Screen Rift valley fever IgM capture ELISA kit [Innovative Diagnostics]) | As per manufacturer's instructions | ||
| Leptospira spp. (Porcine Leptospira Bratislava ELISA, Strip Kit, and Linnodee Bovine Leptospirosis Kit [Linnodee]) | As per manufacturer's instructions | ||
| Toxoplasma spp. (Anti-Toxoplasma gondii IgG Human ELISA Kit [Abcam] and Toxoplasma IgM ELISA [DRG International]) | As per manufacturer's instructions | ||
| Taenia solium | Table S13 | ||
| – Heparinized blood | Bovigam | Mycobacterium spp. (BOVIGAM TB Kit and BOVIGAM TEST KIT (Gamma interferon)[Prionics]) | As per manufacturer's instructions |
| – Whole blood | Microscopy (thick smears) | Trypanosoma spp. | Table S14 |
| Microscopy (thin smears)* | Bacillus anthracis | Table S15 | |
| External parasites | Stored for further testing | ||
| Liver flukes | |||
| Hydatid cysts | |||
| Other cysts | |||
*
This test was only performed in case of a suspect animal or patient.