Figure 1. CD8⁺ T cells stimulated with high or low affinity ligands can form lung T_RM.

CD45.1⁺ OT-I T cells were transferred into CD45.2⁺ hosts and were infected with 40 PFU of IAV_N4, IAV_Y3 or IAV_T4. Spleen and lungs were harvested after anti-CD8α iv injections. (A-D) The number of OT-I T cells, either total (iv⁺ and iv⁻ combined), iv⁺, or iv⁻ cells in the spleen (A, C) and lung (B, D) from mice infected IAV_N4, IAV_Y3, IAV_T4, or IAV_N4 with 1000 OT-I precursors at 10 (A, B) or 34 (C, D) dpi. (E) The number of iv⁻ OT-I T cells in the lung at 34 dpi was divided by the average number of total OT-I T cells in the lung at 10 dpi. (F) The number of iv⁻ OT-I T cells in the lung at 34 dpi was divided by the number of total OT-I T cells in the spleen at 34 dpi, for each mouse. (G) The number of iv⁻ OT-I T cells in the spleen at 34 dpi was divided by the number of total OT-I T cells in the spleen at 34 dpi, for each mouse. The results are compiled from at 2–5 independent experiments with at least 4 mice per group, per experiment (± SEM). A-D one-way ANOVA (see table S1). E-G *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (unpaired t test).