Figure 3. TCR signal strength alters T_RM formation in a distal uninfected mucosal tissue.

Challenged mice were generated as in Figure 1. On indicated dpi, FRTs were harvested after anti-CD8α iv injections. (A, B) The number of endogenous (CD45.1⁻ CD45.2⁺) CD8⁺ H-2D^b-PA₂₂₄/NP₃₆₆ tetramer⁺ CD44^hi cells, either total, iv⁺, or iv⁻ cells in the FRT from mice infected with IAV_N4 or IAV_T4 at 10 (A) or 34 (B) dpi. (C, D) The number of OT-I T cells in the FRT at 10 (C) or 34 (D) dpi. (E) The number of iv⁻ OT-I T cells in the FRT at 34 dpi was divided by the average number of total OT-I T cells in the FRT at 10 dpi. The results (A-E) are representative of 2 independent experiments with at least 3 mice per group (± SEM). *p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 (unpaired t test).