Figure 5. Phenotype of lung T_RM cells is independent of TCR signal strength.

Challenged mice were generated as in Figure 1. (A) Representative flow plots of CD69 and CD103 staining on iv⁻ OT-I T cells in the lung and FRT on 10 and 34 dpi from mice. (B) The percentage of iv⁻ OT-I T cells expressing CD103⁺CD69⁻, CD103⁺CD69⁺ or CD103⁻CD69⁺ (C-F) On 34 dpi, lungs (C, D) and spleens (E, F) were harvested and cells were stimulated ex vivo with 1 μg/mL N4 peptide. As a positive control, cells were stimulated with PMA/Ionomycin. (C, E) Representative IFN-γ staining of iv⁻ lung (C) or total spleen (E) OT-I T cells unstimulated, stimulated with PMA/Ionomycin or N4 peptide. (D, F) Percentage of iv⁻ lung (D) or total spleen (F) IFN-γ⁺ OT-I T cells after ex vivo N4 stimulation. The results (B) are combined from 2 independent experiments with at least 3 mice per group, per experiment (± SEM). The results (D,F) are 1 representative experiment of 2 independent experiments with at least 5 mice per group, per experiment (± SEM). *p < 0.05, ** p < 0.01 (unpaired t test).