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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Circulation. 2019 Jun 7;140(6):487–499. doi: 10.1161/CIRCULATIONAHA.118.038820

Figure 3. Neutropenic mice display diminished Ly6Chi monocyte infiltration and decreased macrophage content after pressure overload.

Figure 3.

a. Scheme of the study. Mice were administered mouse anti-ly6G (500 μg/injection/mouse) or isotype control antibody before and after TAC surgery as indicated. Analysis was performed 2 days after TAC surgery. b. Flow cytometry analysis of cardiac tissue from TAC-treated mice subjected to anti-Ly6G or isotype control antibody (n=7 in each group). Numbers of cells are normalized by tissue weight. Statistical analysis was performed using Mann-Whitney U tests. c. Analysis of transcript expression in the myocardium obtained from sham or TAC-treated mice subjected to anti-Ly6G or isotype control antibody. 36b4 was used as a reference for normalization (n=6 in each group). Statistical analysis was performed using Kruskal-Wallis test with Dunn’s multiple comparison tests (Il1b, Il6, Cxcl1, Cxcl5 and Ccl2) and one-way ANOVA with Tukey’s multiple comparison tests (Cxcl2). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. TAC= transverse aortic constriction.