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. Author manuscript; available in PMC: 2020 Aug 6.
Published in final edited form as: Circulation. 2019 Jun 7;140(6):487–499. doi: 10.1161/CIRCULATIONAHA.118.038820

Figure 7. Myeloid-specific Wnt5a overexpression promotes cardiac hypertrophy and dysfunction in response to pressure overload.

Figure 7.

a. Representative images of hematoxylin-eosin staining of heart sections from Myelo-TG (Rosa-Wnt5a, LysMCre/+) and littermate control (LysMCre/+) mice after TAC. Samples were collected as indicated time points. Scale bar indicates 1 mm. b. Heart or lung weights were adjusted by tibia length. (n=5–10 in each group). Statistical analysis was performed using two-way ANOVA with Sidak’s multiple comparison tests. c. Sequential echocardiographic analysis of Myelo-TG and littermate control mice after TAC at indicated time points (n=10 in each group). Statistical analysis was performed using two-way repeated measures ANOVA with Sidak’s multiple comparison tests. d. Myocyte cross-sectional area was evaluated from the analysis of wheat germ agglutinin staining of the heart sections from each group at the end of study (n=4 in sham and n=6 in TAC groups). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison tests. e. Representative data images and analysis of Picrosirius red staining of heart sections from each group of mice to assess fibrosis (n=4 in each group). Scale bar indicates 100 μm. Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparison tests. *p<0.05, **p<0.05, ***p<0.001, ****p<0.0001. Myelo-TG= myeloid-specific wnt5a overexpression mice, HW= heart weight, LW= lung weight, TL= tibia length, LVPWTd= left ventricle posterior wall thickness at diastole, LVDd= left ventricle diameter at diastole, LVDs= left ventricle diameter at systole, FS= fractional shortening, CSA= cross sectional area of myocyte.