| Chemicals, Peptides, and Recombinant Proteins |
| DMEM medium |
Corning cellgro |
10–013-CV |
| Mammalian cell culture medium |
Expression System |
98–001-01 |
| SF-900 III SFM medium |
GIBCO |
12658–027 |
| Penicillin/Streptomycin |
Corning |
30–002-CI |
| Fetal bovine serum |
AXENIA BIOLOGIX |
F001 |
| n-Dodecyl-b-D-Maltopyranoside (DDM) |
Anatrace |
D310 |
| Cholesteryl hemisuccinate |
Anatrace |
CH210 |
| Digitonin |
AppliChem |
A1905 |
| Protease inhibitor cocktail |
Roche |
18970600 |
| DNase |
Roche |
10104159001 |
| Lipofectamine 2000 transfection reagent |
Invitrogen |
11668019 |
| Critical Commercial Assays |
| CNBR-activated Sepharose beads |
GE Healthcare |
17–0430-01 |
| Superdex 200, 10/300 GL |
GE Healthcare |
17–5175-01 |
| Deposited Data |
| TMEM16F in Digitonin with calcium bound |
This paper |
EMPIAR-10278 |
| TMEM16F in Digitonin without calcium bound |
This paper |
EMPIAR-10279 |
| TMEM16F in Nanodisc |
This paper |
EMPIAR-10280 |
| Cryo-EM structure of TMEM16F in digitonin with calcium bound |
This paper |
PDB: 6P46 and EMD: 20244 |
| Cryo-EM structure of TMEM16F in digitonin without calcium |
This paper |
PDB: 6P47 and EMD: 20245 |
| Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl1 |
This paper |
PDB: 6P48 and EMD: 20246 |
| Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2 |
This paper |
PDB: 6P49 and EMD: 20247 |
| Experimental Models: Cell Lines |
| xSf9 |
ATCC |
CRL-1711 |
| HEK293S GnTI |
ATCC |
CRL-3022 |
| Software and Algorithms |
| RELION |
Scheres, 2012 |
http://www2.mrc-lmb.cam.ac.uk/relion |
| UCSF Chimera |
Pettersen et al., 2004 |
http://www.cgl.ucsf.edu/chimera |
| PHENIX |
Adams et al., 2010 |
https://www.phenix-online.org |
| COOT |
Emsley et al., 2010 |
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Caver |
Pavelka et al., 2016 |
https://www.caver.cz/ |
| PyMOL |
Molecular Graphics System, Version 1.8 Schrodinger, LLC |
https://pymol.org/2/ |
| cisTEM |
Grant et al., 2018 |
https://cistem.org/ |
| Other |
| Quantifoil R1.2/1.3 400 mesh Cu holey carbon grids |
SPI supplies |
4240C-XA |
