Skip to main content
. 2019 Jul 15;11(7):4290–4302.

Figure 3.

Figure 3

miR-338-3p was a direct target of DSCAM-AS1 in HCC cells. A. Complementary sequences between miR-338-3p and DSCAM-AS1 were found using publicly available algorithms. The DSCAM-AS1 mutant sits (MT) is also shown. B. Relative luciferase activity was determined by luciferase reporter assays in SMMC-7721 cells co-transfected with WT-DSCAM-AS1 or MT-DSCAM-AS1 and miR-338-3p or miR-NC. C. The interaction between miR-338-3p and DSCAM-AS1 was determined with RIP assay. D. The expression of miR-338-3p was examined in SMMC-7721 cells transfected with sh-NC and sh-DSCAM-AS1 by qRT-PCR. E. The expression of DSCAM-AS1 was examined in SMMC-7721 cells transfected with miR-338-3p mimics or miR-NC by qRT-PCR. F. qRT-PCR analysis was performed to examine the expression of miR-338-3p in HCC tissues and adjacent normal tissues. G. The expression of miR-338-3p was detected in 4 HCC cell lines (HepG2, Hep3B, Huh7 and SMMC7721) and normal human liver cells (LO2). H. The correlation between DSCAM-AS1 and miR-338-3p was analyzed by Pearson’s correlation analysis. *P<0.05, **P<0.01.