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. 2019 Jul 15;11(7):4337–4347.

Figure 6.

Figure 6

The effect of NAC on senescence-associated secretory phenotype(SASP) in ORX mice. Representative micrographs of paraffin sections of tibias from 16-week-old mice of each group stained immunohistochemically for (A) IL-1α, (D) IL-1β and (G) TNF-α. The percentages of (B) IL-1α-positive, (E) IL-1β-positive and (H) TNF-α-positive cells were determined by image analysis. Real-time RT-PCR was performed on extracts of tibias from 16-week-old mice of each group to investigate gene expression of (C) IL-1α, (F) IL-1β and (I) IL-6. Messenger RNA expression, assessed by real-time RT-PCR analysis, was calculated as a ratio to the GAPDH mRNA level and shown relative to levels in sham mice. Data are presented as the mean ± SEM of determinations, each data-point is the mean of five specimens. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham mice. #P < 0.05, ##P < 0.01 versus ORX mice. (A, D, G) Magnification, 400×.