The effects of downstream Rho GTPase signaling molecules on ADSCs, actin stress fiber formation and migration. A. Transwell assay and quantification of ADSCs treated with PBS, PRP, PRP + ZCL278, PRP + NSC23766, PRP + CT04, PRP + Y27632 or PRP + FRAX597 (n = 6). B. Phallotoxin staining (phalloidin) was performed to stain actin in ADSCs treated with PBS, PRP, PRP + ZCL278, PRP + NSC23766, PRP + CT04, PRP + Y27632 or PRP + FRAX597; nuclei were counterstained with Hoechst 33258 (n = 6). C. Scratch wound assay and quantification of ADSCs treated with PBS, PRP, PRP + ZCL278, PRP + NSC23766, PRP + CT04, PRP + Y27632 or PRP + FRAX597 (n = 6). *P < 0.05, **P < 0.01.