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Swim training-induced MIF decreasing prevents Akt activation caused by HFD in liver. A. The protein levels of pAkt, Akt, pGSK-3β, GSK-3β, and GAPDH (as loading control) in liver tissues (n=6 for each group; *P < 0.05 vs. WT sed). B. Western blot shows that recombinant mouse MIF (rmMIF, 50 ng/ml, 6 hr) induces Akt pathway activation in hepatocyte isolated from HFD mice (n=6 for each group; *P < 0.05 vs. WT mock).
