Figure 2.

B7-H3 increases the radioresistance of gastric cancer cells via DNA double-strand breaks and cell cycle progression. Western blot analysis was used to examine whether the overexpression of B7-H3 inhibited the formation of nuclear γ-H₂AX foci induced by irradiation at 0, 2, 4, 6 and 8 h (A). γ-H₂AX foci formation after 8 Gy X-ray irradiation was significantly inhibited by the overexpression of B7-H3, especially at 4 h. (B & C). Radiation plus B7-H3 overexpression can cause cells to enter S-phase arrest at 12 h (D & E).