Figure 4.

B7-H3 and LC3 expression in gastric cancer cells and tissue samples. A. B7-H3 and autophagy protein expression in mock-, LV-NC- and LV-B7-H3-infected 7901 cells. B7-H3 overexpression downregulated the autophagy proteins LC3, Atg5 and Beclin-1 expression. After 8 Gy X-ray irradiation, protein expression levels were not significantly changed. B. B7-H3 negative and positive and LC3-B negative and positive expression (×200 magnification) in gastric cancer. C. B7-H3 expression is negatively correlated with LC3-B expression in gastric cancer tissue samples (P<0.001). D. Autophagosomes detected by transmission electron microscopy. Mock-, LV-NC- and LV-B7-H3-infected 7901 cells were processed and observed under a transmission electron microscope (2500× & 8000×)