Figure 5.
B7-H3 increases the radiotherapy resistance of gastric cancer cells through regulating baseline levels of cell autophagy. A. The upregulation of baseline autophagy levels in B7-H3 cells by rapamycin can make them sensitive to radiation. B, C. Apoptosis was significantly lower in 7901-LV-B7-H3 + rapamycin cells after radiation than in 7901 LV-B7-H3 and 7901-LV-B7-H3 + DMSO cells after radiation (****P<0.0001). D. The upregulation of baseline autophagy levels in cells overexpressing B7-H3 promoted the formation of nuclear γ-H2AX foci induced by irradiation. E, F. Immunofluorescence was used to confirm the formation of γ-H2AX foci in the nuclei.