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3-MA enhanced VSMCs foam cell formation by inhibition of miR-223-induced autophagy. A-C. LC3II/LC3I ratio and beclin-1 level were decreased whereas the level of p62 was increased by 3-MA, as determined by western blot analysis (n = 5). D and E. Lipid droplet accumulation and intracellular cholesterol contents measurement (n = 4 for both) in ox-LDL-treated VSMCs treated with negative control (50 nmol/L), miR-223 mimic (50 nmol/L) or 3-MA (5 mmol/L). *P < 0.05, **P < 0.01, ***P < 0.001.
