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. 2019 Jul 31;10:518. doi: 10.3389/fendo.2019.00518

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Copyright © 2019 Zayed, Qi and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of several miRNAs differentially expressed between stage IIIa and IIIb follicular cells. (A) miRNA expression levels of dre-miR-22a-3p, dre-miR-29a, dre-miR-181a-3p, and dre-miR-16a detected by the small RNA-seq. (B) Relative expression levels of the same miRNAs measured by real-time PCR. Data represent mean ± SEM (n = 3). ^*P ≤ 0.05; ^**P < 0.01; ^***P < 0.001 as analyzed by Student's t-test.