Skip to main content
. 2019 Jul 31;10:807. doi: 10.3389/fneur.2019.00807

Figure 2.

Figure 2

Effect of hyperhomocysteinemia and high fat diet on liver (A) SGPL1 mRNA, (B) SPTlc2 mRNA, (C) SPTlc2 protein, (D) Cers2 hypothalamus mRNA, (E) Sphk2 hypothalamus mRNA, and (F) SPTlc1 hypothalamus mRNA level in wild type (Cbs+/+ mice) and mice heterozygous for targeted disruption of the Cbs (Cbs+/− mice) on a methionine enriched diet (Met) and high fat diet (HFD). Relative liver and hypothalamus mRNA expression was described in Table 1. Liver SPTlc2 was determined by western blot and quantified by slot blotting [1/1,000 (101)]. β-actin (1/10,000) (Sigma-Aldrich, France) was used as an internal control for Western blot analysis or ponceau-S coloration for slot blot analysis. Data are presented as mean ± SEM, and analyzed with the Student's t-test by using Statview software. n, number of mice. Data were considered significant when p < 0.05.