Table 1.

Relative liver and hypothalamus mRNA expression based upon Q-PCR data obtained from wild-type (Cbs^+/+ mice) and mice heterozygous for targeted disruption of the Cbs (Cbs^+/− mice) on a methionine enriched diet (Met) and high fat diet (HFD).

mRNA (%)	Hypothalamus Cbs+/+ mice Met/HFD (n = 4)	Hypothalamus Cbs+/− mice Met/HFD (n = 4)	Liver Cbs+/+ mice Met/HFD (n = 4)	Liver Cbs+/− mice Met/HFD (n = 4)
Cers1	100 ± 52.2	354 ± 211	100 ± 71	107.7 ± 52.8
Cers2	104 ± 8	247 ± 29**	100 ± 21	124 ± 15
Cers3	100 ± 26	115 ± 48	100 ± 41	95 ± 24
Cers4	99.9 ± 63.9	103.1 ± 58.8	100.9 ± 29.3	101.7 ± 55.1
Cers5	100 ± 18	226 ± 87	100 ± 28	189 ± 43
Cers6	100 ± 33	157 ± 58	100 ± 30	82 ± 21
CERT	100.4 ± 22.5	40.8 ± 17.1	100 ± 35.2	48.2 ± 25.4
DEGS1	100 ± 38.9	61.1 ± 26.1	100 ± 10.3	105.8 ± 29.6
SGPL1	100 ± 51.6	57.3 ± 23	100 ± 15.3	13.5 ± 7.4**
Sphk1	99.3 ± 27.9	71.6 ± 39.2	100.4 ± 44	21 ± 10
Sphk2	100.1 ± 36.4	14.8 ± 6.5*	100.4 ± 24.2	45 ± 21.6
SPTIc1	100 ± 28	10.5 ± 1*	100 ± 22.9	115.4 ± 32.3
SPTIc2	100 ± 2	52 ± 20.6	100 ± 31.6	9.5 ± 6.3*
SPTIc3	98.3 ± 47	371.3 ± 118.3	97.5 ± 24.9	294.5 ± 144.6

Total RNA was isolated from the hypothalamus and liver, reverse transcribed and real time quantitative PCR amplification reactions were carried as described using the LightCycler FastStart DNA Master plus SYBR Green I kit (Roche) (97). The mRNA transcript level was normalized against the mean of two genes: H1a and TBP (Table S1). Data were normalized to the mean of Cbs^+/+ mice on Met/HFD. Data are presented as mean ± SEM, and analyzed with the Student's t-test by using Statview software. n, number of mice.

^*p < 0.05;

^**p < 0.01. Data were considered significant when p < 0.05.