Table 1. Primers used for qPCR.
Target gene | Sense 5′–3′ | Antisense 5′–3′ |
---|---|---|
Nrf2 | GCTGATGGAGTACCCTGAGGCTAT | ATGTCCGCAATGGAGGAGAAGTCT |
HO-1 | TGCCAGTGCCACCAAGTTCAAG | TGTTGAGCAGGAACGCAGTCTTG |
NQO1 | GGAGACAGCCTCTTACTTGCCAAG | CCAGCCGTCAGCTATTGTGGATAC |
GCLC | TGAGATTTAAGC CCCCTCCT | TTGGGATCAGTCCAGGAAAC |
GSTA2 | TCAGTAACCTGCCCACAGTGAAG | GCATGTTCTTGACCTCTATGGCTGG |
UGT1A1 | CACGCTGGGAGGCTGTTAGT | CACAGTGGGCACAGTCAGGTA |
UGT1A6 | CACGTGCTACCTAGAGGCACAG | GACCACCAGCAGCTTGTCAC |
UGT1A9 | GAAGAACATGCATTTTGCTCCT | CTGGGCTAAAGAGGTCTGTCATAGTC |
SULT1A1 | CCCGTCTATGCCCGGATAC | GGGCTGGTGTCTCTTTCAGAGT |
SULT2A1 | TAGGGAAAAATTTAGGGCCAGAT | TTGTTTTCTTTCATGGCTTGGA |
CYP2E1 | CACCGTTGCCTTGCTTGTCTG | CTCATGAGCTCCAGACACTTC |
GAPDH | ACATGGCCTCCAAGGAGTAAGA | GATCGAGT TGGGGCTGTGACT |
Abbreviations: GCLC, glutamate-cysteine ligase catalytic subunit; GSTA2, glutathione S-transferase A 2; HO-1, heme oxygenase-1; Nrf2, nuclear factor erythroid 2-related factor 2; NQO1, NAD(P)H: quinone oxidoreductase 1.