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. 2019 Jul 31;10:1718. doi: 10.3389/fmicb.2019.01718

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Copyright © 2019 Wettstadt, Wood, Fecht and Filloux.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VgrG5 mediates delivery of both PldA and PldB. (A) Plots of recovered cfu of prey strains susceptible for PldA delivery upon bacterial competition assays. Prey strains are as described in Figure 1. PAO1ΔrsmA attacking strains either lacked (Δ) vgrG4b, vgrG5 or both, or expressed vgrG5⁶²⁸ in trans (+). Spots were incubated for 24 h at 25 ^∘C in a 1:1 ratio. One-Way ANOVA analysis with Dunnett’s multiple comparisons test was conducted on the data set obtained from recovered prey on their own with ^*⁣*⁣**p < 0.0001. (B) Representative figure of western blot from a secretion assay using PAO1ΔrsmA. The absence (Δ) of the native vgrG5 or vgrG4b is shown as well as when expressing an HA-tagged version of vgrG5⁶²⁸ in trans (+). Antibodies (from top to bottom) against the C-terminal portion of VgrG4b (VgrG4b), HA, RpoB and Hcp2 were used as indicated on the right.