Figure 5.

First incomplete moment over time. (a–d) Curves in the LFIM may only be visible after sufficient time has passed, allowing both fast- and slow-growing clones to reach large enough sizes to be detectable through sequencing. Examples of the first incomplete moment for a simulation of non-neutral competition are shown for four timepoints. One per cent of mutations are non-neutral with a fitness drawn from a normal distribution, N ∼N (mean = 0.1, std = 0.1). The vertical dashed line shows the detection limit, arbitrarily set at 100 cells. The section of the first incomplete moment that would not be visible due to the detection limit is shown in grey to the left of the line; the visible section is shown in black. The red dashed line is a straight-line fit to the smallest 75% of visible mutant clones. (Online version in colour.)