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Evaluation of modified oligonucleotides as substrates for SNM1A. a) Modified 3’‐Cy3 labelled oligonucleotides are hydrolyzed if binding to the active site in a similar manner to oligonucleotides containing a 5’‐phosphate group. b) Digestion of oligonucleotides 1 a‐5 a (0.8 pmol, 80 nM) by SNM1A (25 fmol, 2.5 nM) after 60 min. nt=nucleotides.
