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. 2018 Dec 4;3(45):12824–12829. doi: 10.1002/slct.201803375

Figure 3.

Figure 3

Evaluation of modified oligonucleotides as inhibitors of SNM1A. a) Modified oligonucleotides act as inhibitors if binding to the metal center in the active site, preventing the hydrolysis of 3’‐Cy3‐labelled 5’‐phosphorylated oligonucleotide 5 a. b) Digestion of oligonucleotide 5 a (0.8 pmol, 80 nM) by SNM1A (25 fmol, 2.5 nM) in the presence of oligonucleotides 1 b‐4 b or 6 b (0.8 pmol, 80 nM) after 60 min. c) Digestion of oligonucleotide 5 a by SNM1A (25 fmol, 2.5 nM) in the presence of different amounts of oligonucleotide 4 b or 6 b relative to 5 a (0.8 pmol, 80 nM) after 60 min with 5 min preincubation. d) Digestion of oligonucleotide 5 a by SNM1A (25 fmol, 2.5 nM) in the presence of 3.3 equivalents of oligonucleotide 4 b or 6 b (2.7 pmol, 264 nM) relative to 5 a (0.8 pmol, 80 nM) after varying incubation times with 5 min preincubation. nt = nucleotides.