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. 2019 Jul 16;11(8):e10058. doi: 10.15252/emmm.201810058

Figure 4. Treatment with multikinase inhibitors results in cell death.

Figure 4

  • A
    Viability of IBL‐202‐ or IBL‐301‐treated cells determined by CellTiter‐Glo. Values are reported as mean ± SEM. n = 2 replicates for LU‐NB‐2 and LU‐NB‐3, n = 3 replicates for SK‐N‐BE(2)c and SK‐N‐SH.
  • B, C
    Cell cycle distribution of LU‐NB‐3 cells determined by flow cytometry. Bars show mean values from two independent experiments.
  • D
    Cleaved caspase‐3 (Cl Casp3) levels in LU‐NB‐3 cells determined by Western blotting. SDHA levels were used as loading control.
  • E
    Flow cytometry analyses of Annexin V and PI stainings following treatment with 360 nM IBL‐202 or 50 nM IBL‐301.
  • F
    Quantification of live and dead cells from the Annexin V/PI stainings. Dead cells = PI positive, live cells = PI negative. Dot plots from two independent experiments and error bars represent SEM.

Source data are available online for this figure.