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. 2019 Jul 31;10:1767. doi: 10.3389/fimmu.2019.01767

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Copyright © 2019 Holl, Frazier, Landa, Beasley, Hwang and Nair.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dendritic cell analysis in peripheral blood of patients with cancer. Non-single events were excluded as described in Figure 1. (A) melanoma; (B) breast cancer; (C) brain cancer. DCs were identified based on expression of HLA-DR and lack of lineage (Lin) markers (CD3, CD14, CD19, CD20, CD56). Myeloid DCs (mDCs) were identified as Lin-HLADR+CD11c+. All mDCs were further divided CD11c+CD1c+CD16– mDC1, CD11c+Clec9A+ CD16– mDC2 and CD11c+CD16+Clec9A– inflammatory mDCs. pDCs were identified as HLADR+CD11c–CD123+. Range in frequency of cell subsets is shown in Table S7. DC, dendritic cell; mDC, myeloid dendritic cell; pDC, plasmacytoid dendritic cell.