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Immune cell analysis in melanoma tumor tissue. Melanoma tumors were gated on live cells (PI-negative). Immune cell analysis was then carried out as described in Figure 1. (A) Basic immune cell analysis; (B) T cell subset analysis; (C) Treg analysis. (D) B cell analysis. Range in frequency of cell subsets is shown in Tables S8, S9. PI; propidium iodide. CM, Central Memory; E, effector; EM, Effector Memory; N, naïve.
