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. 2019 Jul 2;11(8):e9903. doi: 10.15252/emmm.201809903

Figure 2. Exposure to ROS promotes cytokine secretion from human macrophages and from PBMC .

Figure 2

  • A–E
    Cytokine secretion of macrophages (A–D) or PBMC (E) incubated with the indicated stimuli for 24 h. RBC, iRBC, RBCL, iRBCL, XO, hypoxanthine (Hyx), the anti‐oxidants N‐acetyl‐l‐cysteine (NAC), and 1‐thioglycerol (1‐TG), and heat inactivated (HI) XO were added to macrophages for 24 h. Febuxostat (Feb) was pre‐incubated for 30 min with XO before addition to macrophages. Each symbol represents the value obtained for cells from an independent donor in an independent experiment. Insets show paired samples by donor in two different conditions. Blue asterisks indicate significance when values were compared with RBCL (A, E) or XO (B–D). Gray asterisks mark comparison with control (B–D). (A) n = 7; (B–E) n = 3. One‐way ANOVA with Tukey test for multiple comparisons was performed to determine statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001).

Source data are available online for this figure.