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. 2019 Jul 30;15:1744806919866529. doi: 10.1177/1744806919866529

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Effects of EA on the increased expression of Iba1 and c-fos in the L4–L5 spinal cord induced by formalin injection (n = 12). (a) Representative bands and quantification of the Western blot analysis revealed that EA treatment dramatically reduced the increased Iba1 protein level. (b) Representative bands and quantification of Western blot analysis revealed that EA significantly reduced the increased c-fos protein level. (c) Representative immunostaining pictures revealed the change in Iba1 expression in the L4–L5 spinal cord in control, model, pre-EA, and post-EA group. (d) Representative immunostaining images revealed the change in c-fos expression in the L4–L5 spinal cord in control, model, pre-EA, and post-EA group. ^#P<0.05, compared with control group; *P<0.05, compared with model group, ^&P<0.05, compared with post-EA group; scale bars = 50 μm. EA: electroacupuncture; Iba1: ionized calcium binding adapter molecule 1.