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. 2019 Jul 31;10:1773. doi: 10.3389/fimmu.2019.01773

Figure 4.

Figure 4

Effect of 2′-FL on the fecal microbiota composition on day 8. The sequencing of the amplicon targeting the V3–V4 region of the 16S rRNA was performed following the 16S Metagenomic Sequencing Library Illumina 15044223 B protocol. The relative proportion of the bacteria was calculated for (A) phylum, family, genus, and species. (B) PCA of the proportion of bacteria at the level of family. (C) Venn Diagrams showing the number of families and genera in the REF and 2′-FL groups. Results are expressed as the mean value (n = 3/group, corresponding to one random animal/litter). *p < 0.05 compared to REF group (by Mann–Whitney U-test). REF, Reference; 2′-FL, 2′-fucosyllactose.