Table 5.
Frequency and distribution of mating-type (MAT) genes among isolates of Aspergillus flavus in soil from maize fields in southeastern United States treated with Afla-Guard and AF36 biocontrol strains in 2012.
| Mating-type frequencyc | |||||
|---|---|---|---|---|---|
| State | Sampling perioda | Genetic scaleb | MAT1-1 | MAT1-2 | P-valued |
| Alabama | Pre-application | Corrected | 80.8 (21) | 19.2 (5) | 0.0025 |
| Uncorrected | 36.4 (32) | 63.6 (56) | 0.0138 | ||
| Post-application | Corrected | 52.8 (19) | 47.2 (17) | 0.8679 | |
| Uncorrected | 38.8 (33) | 61.2 (52) | 0.0503 | ||
| Harvest | Corrected | 59.1 (13) | 40.9 (9) | 0.5235 | |
| Uncorrected | 24.4 (20) | 75.6 (62) | 0.0001 | ||
| Georgia | Pre-application | Corrected | 55.2 (16) | 44.8 (13) | 0.7111 |
| Uncorrected | 75.0 (63) | 25.0 (21) | 0.0001 | ||
| Post-application | Corrected | 62.1 (18) | 37.9 (11) | 0.2649 | |
| Uncorrected | 69.8 (60) | 30.2 (26) | 0.0001 | ||
| Harvest | Corrected | –c | –c | – | |
| Uncorrected | –c | –c | – | ||
| North Carolina | Pre-application | Corrected | 41.9 (13) | 58.1 (18) | 0.4731 |
| Uncorrected | 25.6 (21) | 74.4 (61) | 0.0001 | ||
| Post-application | Corrected | 34.6 (9) | 65.4 (17) | 0.1686 | |
| Uncorrected | 14.6 (13) | 85.4 (76) | 0.0001 | ||
| Harvest | Corrected | 39.3 (11) | 60.7 (17) | 0.3449 | |
| Uncorrected | 23.2 (19) | 76.8 (63) | 0.0001 | ||
Denotes when soil samples were collected from the field in relation to the application of the biocontrol agents. Afla-Guard and AF36 were evaluated in both years in North Carolina. In Alabama, Afla-Guard was evaluated in both years, while AF36 was evaluated only in 2013. In Georgia, only Afla-Guard was evaluated in both years.
Mating-type designation based on either uncorrected or clone corrected multilocus haplotype data.
Numbers presented in parentheses refer to number of isolates examined. Soil samples were not collected at harvest in Georgia.
Probability from a two-tailed exact binomial test performed under the null hypothesis of no significant difference in the frequency of isolates with MAT1-1 and MAT1-2 genes.