Table 6.
Frequency and distribution of mating-type (MAT) genes among isolates of Aspergillus flavus in soil from fields in the southeastern United States treated with Afla-Guard and AF36 biocontrol strains in 2013.
| Mating-type frequencyc | |||||
|---|---|---|---|---|---|
| State | Sampling perioda | Genetic scaleb | MAT1-1 | MAT1-2 | P-valued |
| Alabama | Pre-application | Corrected | 19.4 (13) | 80.6 (54) | 0.0031 |
| Uncorrected | 22.5 (18) | 77.5 (62) | 0.0001 | ||
| Post-application | Corrected | 53.1 (17) | 46.9 (15) | 0.8601 | |
| Uncorrected | 27.7 (23) | 72.3 (60) | 0.0001 | ||
| Harvest | Corrected | 38.6 (17) | 61.4 (27) | 0.1742 | |
| Uncorrected | 25.9 (21) | 74.1 (60) | 0.0001 | ||
| Georgia | Pre-application | Corrected | 40.5 (15) | 59.5 (22) | 0.3240 |
| Uncorrected | 53.0 (44) | 47.0 (39) | 0.6609 | ||
| Post-application | Corrected | –c | –c | – | |
| Uncorrected | –c | –c | – | ||
| Harvest | Corrected | 25.0 (1) | 5.0 (3) | 0.6250 | |
| Uncorrected | 1.2 (1) | 98.8 (85) | 0.0001 | ||
| North Carolina | Pre-application | Corrected | 56.7 (17) | 43.3 (13) | 0.5847 |
| Uncorrected | 50.0 (40) | 50.0 (40) | 1.0001 | ||
| Post-application | Corrected | 65.7 (23) | 34.3 (12) | 0.0895 | |
| Uncorrected | 47.6 (39) | 52.4 (43) | 0.7407 | ||
| Harvest | Corrected | 56.0 (14) | 44.0 (11) | 0.6900 | |
| Uncorrected | 32.5 (26) | 67.5 (54) | 0.0023 | ||
Denotes when soil samples were collected from the field in relation to the application of the biocontrol agents. Afla-Guard and AF36 were evaluated in both years in North Carolina. In Alabama, Afla-Guard was evaluated in both years, while AF36 was evaluated only in 2013. In Georgia, only Afla-Guard was evaluated in both years.
Mating-type designation is based on either uncorrected or clone corrected multilocus haplotype data.
Numbers presented in parentheses refer to number of isolates examined. Soil samples were not collected at post-application of the biocontrol agent harvest in Georgia.
Probability from a two-tailed exact binomial test performed under the null hypothesis of no significant difference in the frequency of isolates with MAT1-1 and MAT1-2 genes.