. 2019 Jul 31;10:1738. doi: 10.3389/fmicb.2019.01738

Table 7.

Neutrality based on Fu (F_S) and Tajima (D) tests and nucleotide diversity estimates (π) for the three multilocus sequence typing loci for populations of Aspergillus flavus collected from fields in the southeastern United States treated with Afla-Guard and AF36 biocontrol strains.

State	Sampling period	trpC^a			mfs^a^,^b			AF17^a
		F_S	D	π	F_S	D	π	F_S	D	π
Alabama	Pre-application	−1.0786	−0.7180	0.0011	−1.6917	−0.7877	0.0058	−0.4803	−0.5979	0.0116
	Post-application	−0.9824	−0.6453	0.0011	−2.0735	−0.9052	0.0048	0.9633	0.9390	0.0114
	Harvest	−1.3830	−0.7248	0.0017	−3.6820	−0.8408	0.0054	2.3024	1.0782	0.0099
	2012	−1.1333	−0.7239	0.0003	−3.7406	−0.9739	0.0042	0.2268	0.5271	0.0092
	2013	−3.0801	−1.0600	0.0020	−3.3376	−0.7285	0.0055	0.5519	−0.7717	0.0085
Georgia	Pre-application	−1.8387	−0.8155	0.0017	−1.6175	−0.3810	0.0042	−0.0881	−0.3469	0.0050
	Post-application	−1.6738	−0.8295	0.0011	−2.5014	−0.7743	0.0041	−0.3985	−0.1441	0.0044
	Harvest	−1.9450	−0.9182	0.0010	−4.0554	−1.1048	0.0036	0.1461	0.0804	0.0038
	2012	−1.2246	−0.6330	0.0013	−2.3542	−0.9817	0.0033	−0.4891	−0.4299	0.0052
	2013	−1.1754	−0.6162	0.0013	−2.6525	−0.6877	0.0046	0.1260	0.0405	0.0034
North Carolina	Pre-application	1.4768	0.6758	0.0046	−0.4811	−0.0224	0.0042	2.8917	1.7240	0.0100
	Post-application	1.5741	0.9971	0.0057	0.1346	−1.0536	0.0017	1.4757	1.3664	0.0063
	Harvest	−0.9691	−0.7874	0.0002	−1.1847	−1.5218^*	0.0005	−1.1684	−1.2295	0.0012
	2012	2.5226	1.4506	0.0060	−2.0405	−1.0387	0.0023	1.8450	0.9399	0.0079
	2013	−2.7494	−1.3204	0.0011	−0.7275	−0.2841	0.0033	0.0532	−0.2678	0.0046

F_S measures departure from neutrality based on Fu (1997), where negative values are evidence for an excess number of alleles and suggest recent population growth, while positive values are evidence for a deficiency of alleles from a recent bottleneck; D measures departure from neutrality based on Tajima (1989), where negative values suggest rapid population growth, while positive values indicate population contraction; Nucleotide diversity (π) is based on (Nei and Li, 1979).

The asterisk (^*) denotes values with significant (P < 0.05) deviation from neutrality based on either the F_S or D test.