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. 2019 Aug 6;18:130. doi: 10.1186/s12934-019-1177-y

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Production of NAG by engineered strain from glutamate and acetate. The bioconversion medium used here contained 50 mM sodium glutamate and 100 mM sodium acetate. The bioconversion reactions were performed at 37 °C and 200 rpm. H₂SO₄ was added at different reaction times to adjust the pH value