Fig. 6.

Comparison of glucose, acetate, and fatty acid as source of acetyl-CoA. The reaction mixture containing 50 mM sodium glutamate and 1 × M9 salts buffer were used. a Glucose (80 mM) was supplemented at rates of 20, 20, 20, 10, and 10 mM at 0, 1, 2, 4, and 6 h of bioconversion. b Acetate (160 mM) was supplemented at rates of 30, 30, 30, 30, 20, and 20 mM at 0, 2, 4, 6, 8, and 10 h of bioconversion. Additionally, 2 mM glucose was added at 0 h of bioconversion to supply ATP for the acetylation of acetate. c Palmitic acid (20 mM) was supplemented at 5 mM at each addition at 0, 3, 6, and 9 h of bioconversion