Table 3.
Strains and plasmids used in this study
| Strain/plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F−endA1 glnV44 thi−1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZ ∆M15 ∆(lacZYA-argF)U169, hsdR17(r−K m+K), λ− | Invitrogen |
| E. coli BW25113 | lacIqrrnBT14∆lacZWJ16hsdR514∆araBADAH33∆rhaBADLD78 | Invitrogen |
| N00 | E. coli BW25113, ∆argB, ∆argA | This study |
| N01 | E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf | This study |
| N02 | E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs | This study |
| N03 | E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA | This study |
| N04 | E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA, ∆pta | This study |
| N05 | E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA, ∆gltA | This study |
| N06 | E. coli BW25113, ∆argB, ∆argA, ∆fadR, PfadD::PCPA1 | This study |
| Plasmids | ||
| pRB1 s | RSF1030 origin, Pbad promoter, StrR | Our lab |
| pSB1a | pSC101 origin, Pbad promoter, AmpR | Our lab |
| pN01 | argA from E. coli cloned into pRB1 s | This study |
| pN02 | argA from Pseudomonas aeruginose cloned into pRB1 s | This study |
| pN03 | argA from Xanthomonas campestris cloned into pRB1 s | This study |
| pN05 | argA from Corynebacterium glutamicum cloned into pRB1 s | This study |
| pN06 | argA from Streptomyces coelicolor cloned into pRB1 s | This study |
| pN08 | argA from Mycobacterium tuberculosis cloned into pRB1 s | This study |
| pN14 | argA from Thermus thermophilus cloned into pRB1 s | This study |
| pN18 | argA from Meiothermus ruber cloned into pRB1 s | This study |
| pN19 | argA from Kitasatospora setae cloned into pRB1 s | This study |
| pN20 | argA from Deinococcus deserti cloned into pRB1 s | This study |
| pAcs | acs from E. coli cloned into pSB1a | This study |
| pAck | ackA-pta from E. coli cloned into pSB1a | This study |