Fig. 3.

Sample preparation for cryosectioning. (a) Sample and cryosection specimen holder equilibrating to the temperature of the cryostat. (b) Freezing medium added to the specimen holder is beginning to freeze. (c) Once the freezing medium has turned white, the sample is pressed into it. (d) Cryostat mount before the specimen holder is positioned. Take note of the specimen orientation ball assembly and the specimen orientation handle in black. (e) Mounted specimen holder. (f) The sample in (e) was sectioned through until the flies became visible. This is the point to start collecting sections on a slide