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. Author manuscript; available in PMC: 2019 Aug 7.
Published in final edited form as: Methods Mol Biol. 2019;1889:267–281. doi: 10.1007/978-1-4939-8897-6_16

Fig. 6.

Fig. 6

IFM and TDT dissections. (a) Anesthetized flies on CO2 pad. (b) Anesthetized flies in OCT media. (c) Removal of legs using sharp edge of the needle. (d) Complete removal of legs and wings. (e) Removal of head and abdomen in tissue freezing media using the sharp edge of a needle. (f) Thorax in 1 M Sucrose. (g) Anterior view of the thorax, arrow shows hole where head was attached. (h) Inserting the needle on the anterior side of the thorax to make vertical cut. (i) Bisected thorax. (j) Visible IFMs on bisected thorax. (k) Detaching the IFMs using tip of the needle. (l) IFMs lifted out but still attached to thorax. TDT visible underneath IFMs. (m) Extracted IFMs next to one half of the thorax. (n) Bundle of IFMs. (o) Extracted TDT with dorsal cuticle attached. (p) TDT in 1 M sucrose media. D dorsal, V ventral, A anterior, P posterior