Fig. 7.

Molecular diversity of Drosophila flight and jump muscles revealed with the microsampling technique. Frozen young adults were sectioned and, under microscope control, flight (IFM) and jump (TDT) muscles were scraped and collected for RNA isolation, cDNA was made with random hexamer oligos and equalized cDNA samples were used for end-point amplification of specific gene products using sequence-specific primers. Note that this technique reveals fiber-specific preferences in alternative mRNA splicing of Troponin I transcripts (wupA), as well as mutually exclusive expression of Troponin C genes (TpnC4 and TpnC41C); amplification of pan-specific Myosin Heavy Chain (Mhc) transcripts was used as loading control