Table 1.
Antibodies used for immunocytochemistry
| Target antigen | Antibody species | Vendor | Clone/product number | Dilution |
|---|---|---|---|---|
| Pecam-1 | Rabbit | Thermo Scientific | RB-10333 | 1:25 |
| Glut-1 | Mouse | Thermo Scientific | SPM498 | 1:500 |
| Claudin-5 | Mouse | Life Technologies | 4C3C2 | 1:200 |
| ZO-1 | Mouse | Thermo Scientific | 1A12 | 1:100 |
| VE-Cadherin | Mouse | Santa Cruz | F-8 | 1:100 |
| Occludin | Mouse | Life Technologies | OC-3F10 | 1:50 |
| P-glycoprotein | Mouse | Thermo Scientific | P170(F4) | 1:25 |
| β-tubulin III | Rabbit | Sigma | T3952 | 1:500 |
| GFAP | Rabbit | Dako | ZO334 | 1:500 |
| NG2 | Mouse | Millipore | MAB2029 | 1:100 |
| PDGFR-β | Rabbit | Cell Signaling Technology | 28E1 | 1:100 |