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. 2019 Aug 6;38:341. doi: 10.1186/s13046-019-1349-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Depleting PTOV1 impaired stem cell-like properties in NSCLC cells. a Representative images of tumor sphere formation of the indicated cells. b FACS analysis of the portion of CD133⁺ cells. c Real-time quantitative PCR detecting the expression of the pluripotency factors in the indicated cells. d Real-time quantitative PCR detecting the expression of PTOV1 and CD133 in undifferentiated and serum-induced differentiated cells. Error bars represent the mean ± SD from 3 independent experiments. *, P < 0.05; #, P < 0.01; $, P < 0.001, two-tailed, unpaired t-test