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. 2019 Jul 31;10:993. doi: 10.3389/fpls.2019.00993

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Copyright © 2019 Gurrieri, Distefano, Pirone, Horrer, Seung, Zaffagnini, Rouhier, Trost, Santelia and Sparla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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pK_a determination of the catalytic cysteines of AtAMY3. AtAMY3 was incubated in different buffers ranging from pH 4 to 12 in presence or absence of IAM before measuring the activity. The residual activity at each pH value was calculated as percentage of inhibition between IAM-treated and untreated samples, and expressed as a function of pH. The obtained curve was fitted by non-linear regression with two-pK_a dependence. Results are mean ± SD (n = 3; SD < 10% are omitted for clarity).