Table 1.

Cannabinoid Receptor Binding and Activation of DHA-EA, EPA-EA and oxygenated metabolites

Compound	CB₁ EC₅₀ values (nM)	CB₂ EC₅₀ values (nM)	References
AEA	0.7^a	1352^a	(19)
	39.2 ± 5.7^b	ND^b	(28)
	543 ± 83^b	ND^b	(12)
	160 ± 13^c	ND^c
	89 ± 10^b (with PMSF)	ND^b	(60)
	5400 ± 1600^b	ND^b
DHA-EA	1044^a	305^a	(19)
	9.8^a	ND^a	(20)
	12200 ± 520^b	ND^b	(12)
	6000 ± 800^c
	324.1 ± 9.2^b	ND^b	(28)
19,20 EDP-EA	108^a	280^a	(19)
10,17-diHDHA-EA	ND^a	3.9^a	(20)
15-HEDPEA	ND^a	1^a	(20)
EPA-EA	0.1^a	2.1^a	(19)
	1470 ± 500^b (with PMSF)	ND^b	(60)
	162.3 ± 13.6^b	ND^b	(28)
17,18 EEQ-EA	18.5^a	1.4^a	(19)

Abbreviations: N-docosahexaenoylethanolamine (DHA-EA), eicosapentaenoyl ethanolamide (EPA-EA), epoxydocosapentaenoic acid-ethanolamide (EDP-EA), epoxyeicosatetraenoic acid-ethanolamide (EEQ-EA), dihydroxydocosahexaenoyl ethanolamide (diHDHA-EA), hydroxy-epoxy-docosapentaenoyl ethanolamide (HEDPEA) and not determined (ND).

Experiments were performed with cell cultures transfected with CB receptors

Experiments were performed with displacement of CB receptor agonist

Experiments were measured by inhibition of cAMP