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. 2019 Jul 31;10:1727. doi: 10.3389/fmicb.2019.01727

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Copyright © 2019 Silva, Taira, Dias, Souza, Nosanchuk, Travassos and Taborda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferation of CD4 and CD8 T lymphocytes. Splenocytes from mice infected with Paracoccidioides brasiliensis were incubated with BMDCs (A,B) or MoDCs (C,D) previously pulsed with P10, and after 96 h the proliferation was analyzed by flow cytometry using the CFSE dilution method. Splenocytes cultivated in the absence (splenocytes) or presence of P10 (sple+P10) or co-incubated unprimed DC (BMDC or MoDC) were used as controls. ^*Statistical significance between the splenocyte+DC and splenocyte+DCP10 groups. Data shown are representative of two independent experiments, analyzed by one-way ANOVA followed by Tukey’s post-test, where ^*p < 0.05 and ^∗∗p < 0.01.