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. 2019 Jul 31;10:1727. doi: 10.3389/fmicb.2019.01727

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Copyright © 2019 Silva, Taira, Dias, Souza, Nosanchuk, Travassos and Taborda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine profile in the supernatant of co-culture of splenocytes and BMDC or MoDC pulsed or not with P10. Splenocytes from mice infected with P. brasiliensis were co-cultivated with BMDC or MoDC pulsed (BMDC+P10 or MoDC+P10) or not (BMDC or MoDC) with P10, and after 96 h the measurement of cytokines in the supernatant was analyzed by CBA. Splenocytes cultivated alone (Sple-only) were used as controls. The data are representative of two independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s post-test, where ^*p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, or ^*⁣*⁣**p < 0.0001 in comparison to Sple-only (control culture with DC) and #p < 0.05, ##p < 0.01, ###p < 0.001, or ####p < 0.0001 for comparison of respective BMDC or MoDC groups pulsed or not with P10. (A–H) correspond respectively to cytokines, IL-12p70, IFN-γ, TNF-α, IL-6, IL-2, IL-10, IL-4, IL-17A.